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教授挨拶

椎名教授 島根大学医学部泌尿器科は、島根医科大学として昭和52年に設置され、初代の石部知行教授のもとで泌尿器科学教室としての基礎が築かれました。石部教授の在任期間中に碓井亞先生(当時助教授)が広島大学医学部泌尿器科学教室教授を拝命し転出致しました。石部教授退官後は、井川幹夫教授(現島根大学附属病院院長)が2代目として選出され、手術では会陰式前立腺全摘術や膀胱全摘後の回腸新膀胱造設術、画像診断ではMRIの治療効果判定への応用やtissue elastographyによる「前立腺の硬さ」の客観的評価に基づく前立腺癌の局在診断の向上など、着実に業績が集積されました。2012年10月から椎名浩昭が3代目教授として島根大学医学部泌尿器科学教室を主宰しております。
 島根大学泌尿器科では尿路性器がん、慢性腎不全と腎移植、下部尿路閉塞疾患を中心に腎・尿路・男性生殖器および副腎などの後腹膜臓器の疾患を対象とした疾患の臨床と研究を行っております。高齢化社会の到来に伴い、年齢を考慮した治療戦略が必要と考えられます。初期のがんではQOLを維持しつつ確実な制癌を目指した低侵襲手術が必要で、私たちも昨年12月から低侵襲手術としてロボット支援前立腺全摘を開始し制癌とQoLの両者のバランスを重視した治療を提供しております。特に、ご高齢の患者さんには安全性に加えて確実性も要求されるため、ICG蛍光造影法を応用した術式を確立し、すなわち腎癌では正確な腎部分切除を、前立腺がんや浸潤性膀胱がんでは確実なリンパ節郭清を遂行しています。一方、島根県各地から紹介される進行期がんに対して、拡大手術を核とした集学的治療にも積極的に取り組み、再発癌に対しては新規抗がん剤の導入など積極的な集学的治療に努め予後改善に努力しています。
 末期腎不全に対しては、兵庫県立西宮病院や兵庫医科大学と連携して積極的に腎移植を行なっており、血液型不適合夫婦間腎移植、免疫学的高リスク腎移植、あるいは他院で移植困難と判断された小児腎移植なども行っています。本院は島根県内唯一の献腎移植認定施設であり、腎移植医療の中心としても日々実績を積んでおります。慢性腎不全に関しては、糖尿病性腎症が血液透析導入の原疾患として第1位となり、我が国でも血液透析導入症例も30万人を超え当院でも当科が中心となってより安全で充実した血液浄化治療を提供しています。一方で透析患者さんの高齢化に伴い内シャント造設術が困難な症例に遭遇する機会も増加し、このような症例の血管手術や人工血管グラフト留置術も精力的に行ない、島根県の血液浄化治療のセンター的役割も果たしています。
 当科の主たる研究テーマは、①尿路性器癌の診断と治療、②前立腺の硬度と排尿動態の関連および③膀胱再生です。これらは実際の臨床で遭遇した疑問から研究を計画したものであり、得られた結果を実際の臨床に還元できるよう努めております。臨床で遭遇した小さな疑問点を等閑にしないこと、常に探究心をもつこと、「臨床は厳しく、実験は楽しく、永遠に努力を惜しまない」このような姿勢をもち続け、医局員同士で切磋琢磨しながら、尿路性器癌の分子生物学(geneticsとepigenetics)、画像診断における新たな展開、前立腺の「硬さ」の科学、手術前後の縦断的QoL解析、尿路性器癌の化学療法、膀胱再生など数多くの業績を発信してきました。これらは診療科を超えてあるいは臨床と基礎の垣根を超えて多くの先生方のご支援をうることができた成果であり、大学ならではの賜物と考えられます。
 急速な社会の高齢化に伴い、今後は泌尿器科医の活躍する局面がますます加速的に増加するものと思われます。このような社会情勢の変化とニーズに対処すべく多くの有能な泌尿器科医師を育成し輩出すること、人間として医者として優しい地域に根付く後輩の育成に力を注ぐこと、また泌尿器科医師のネットワークを地域社会に構築し、最先端の泌尿器科医療を広く社会に展開すること、これらが最重要課題であると判断しています。このような環境整備を行いながら、視点を変えたアプローチからの泌尿器科疾患を再考し,チームとしてより優れた医療を臨床に展開できるよう泌尿器科研究を発展させていきたいと考えています。


教授プロフィール

<略歴>


1985年 5月
島根医科大学医学部卒業
1986年 1月
島根医科大学医学部付属病院泌尿器科助手
1991年 4月
島後町村組合立隠岐病院泌尿器科医長
1992年 7月 島根医科大学医学部付属病院泌尿器科助手
1995年 3月
島根医科大学医学部付属病院泌尿器科講師
1997年 1月
島根医科大学医学部泌尿器科助教授
2001年 5月〜2004年 3月 アメリカ カリフォルニア大学サンフランシスコ校留学
(Prof. Emil A Tanagho and Prof. Rajvir Dahiya) 
2004年 5月 島根大学泌尿器科学助教授
2008年 4月 島根大学付属病院血液浄化治療部長 併任
2012年10月 島根大学医学部泌尿器科教授
現在に至る。

<免許資格>
医学博士
日本泌尿器科学会泌尿器科専門医・指導医
日本がん治療認定医機構暫定教育医
日本臨床腎移植学会腎移植認定医
日本移植学会認定医
臨床研修指導医
<専門領域>
尿路性器癌の治療
尿路性器癌の分子生物学
腎移植と慢性腎不全
膀胱再生
<所属学会>
日本泌尿器科学会
泌尿器科再建再生研究会
日本移植学会
日本臨床腎移植学会
腎移植・血管外科研究会
日本アンドロロジー学会
日本透析医学会
中国四国臨床臓器移植研究会
日本泌尿器内視鏡学会
<趣味・特技>
統計解析・BTOコンピューター

教授業績

学術論文

欧文原著

  1. Shiina H, Himeno Y, Ishibe T: Urological complications in gynaecological diseases. Review of 18 cases requiring diversion or reconstruction of the urinary tract. Int Urol Nephrol. 21:511-514, 1989
  2. Shiina H, Ishibe T, Usui T: Study of estramustine binding protein (EMBP): purification of rat EMBP and establishment of RIA. Biochem Biophys Res Commun. 165:947-955, 1989
  3. Shiina H, Mizutani M, Ishibe T: Estramustine binding protein in human benign prostatic hypertrophy. Andrologia 22:309-312, 1990
  4. Shiina H, Sumi H, Ishibe T, Usui T: Relationship of thyroid to estramustine binding protein (EMBP) concentration in rat prostate. Andrologia 23:145-149, 1991
  5. Shiina H, Ishibe T, Usui T: Immunoreactive protein to rat estramustine binding protein in human seminal plasma: its relationship to prostatic acid phosphatase and zinc. Andrologia 23:177-179,1991
  6. Moriyama-Gonda N, Sumi H, Shiina H, Himeno Y, Ishibe T: Unstable chromosome aberrations in bladder and renal cell carcinoma. Cancer Genet Cytogenet. 56:65-72, 1991
  7. Shiina H, Himeno Y, Ishibe T: Organisms in the prostate and antibiotics in the treatment of postoperative infections. Urol Int. 48:187-190, 1992
  8. Shiina H, Ishibe T, Usui T: Interaction of testosterone-oestradiol binding globulin (TeBG) and albumin with human prostatic carcinoma in vitro. Int Urol Nephrol. 25:577-82, 1993
  9. Shiina H, Ehara S, Ishibe T: Vesicourethral function after surgery for uterine cancer: predictive value of postoperative maximum urethral closure pressure on residual urine. Urol Int. 51:125-128, 1993
  10. Shiina H, Sumi H, Ishibe T, Usui T: Study of estramustine binding protein: its relationship to androgen dependency and histological differentiation in human prostatic carcinoma tissue. Urol Int. 52:213-216, 1994
  11. Shiina H, Shirakawa H, Yokogi H, Kishi H, Mizutani M, Igawa M, Ishibe T: Clinical value of argyrophilic organizer region in bladder transitional cell carcinoma. Urol Int. 54:74-79, 1995
  12. Igawa M, Urakami S, Shiina H, Kishi H, Himeno Y, Ishibe T, Kadena H, Usui T: Neoadjuvant chemotherapy for locally advanced urothelial cancer of the upper urinary tract. Urol Int. 55:74-77, 1995
  13. Igawa M, Urakami S, Shiina H, Ishibe T, Kadena H, Usui T: Long-term results with M-VAC for advanced urothelial cancer: high relapse rate and low survival in patients with a complete response. Br J Urol. 76:321-324, 1995
  14. Shiina H, Igawa M, Urakami S, Shirakawa H, Ishibe T: Immunohistochemical analysis of nm23 protein in transitional cell carcinoma of the bladder. Br JUrol. 76:708-713, 1995
  15. Igawa M, Urakami S, Shirakawa H, Shiina H, Ishibe T, Usui T, Moriyama H: A mapping of histology and cell proliferation in human bladder cancer: an immunohistochemical study. Hiroshima J Med Sci. 44:93-97, 1995
  16. Igawa M, Urakami S, Shirakawa H, Shiina H, Ishibe T, Kadena H, Usui T, Kawanishi M: Factors related to the outcome of M-VAC in 101 patients with advanced urothelial cancer. Hiroshima J Med Sci. 44:113-117, 1995
  17. Igawa M, Urakami S, Shiina H, Ishibe T, Shirane T, Usui T, Chodak GW: Immunohistochemical evaluation of proliferating cell nuclear antigen, prostate-specific antigen and alpha 1-antichymotrypsin in human prostate cancer. Br J Urol. 77:107-112, 1996
  18. Urakami S, Igawa M, Shirakawa H, Shiina H, Ishibe T: Biological characteristics of inverted papilloma of the urinary bladder. Br J Urol. 77:55-60, 1996
  19. Shiina H, Igawa M, Ishibe T: Estramustine-binding protein to dihydrotestosterone ratio in human prostatic carcinoma: a new marker for predicting disease progression. Br J Urol. 77:96-101, 1996
  20. Shiina H, Urakami S, Shirakawa H, Shigeno K, Himeno Y, Mizutani M, Igawa M, Ishibe T: Evaluation of the argyrophilic nucleolar organizer region, nuclear DNA content and mean nuclear area in transitional cell carcinoma of bladder using a quantitative image analyzer. Eur Urol. 29:99-105, 1996
  21. Shiina H, Urakami S, Shirakawa H, Igawa M, Ishibe T, Usui T: Estramustine-binding protein in carcinoma and benign hyperplasia of the human prostate. Eur Urol. 29:106-110, 1996
  22. Igawa M, Urakami S, Shirakawa H, Shiina H, Kishi H, Himeno Y, Ishibe T, Kadena H, Usui T: Preliminary results of concurrent methotrexate, cisplatin and radiation treatment for locally advanced urothelial cancers. Int Urol Nephrol. 28:189-194, 1996
  23. Shiina H, Igawa M, Yagi H, Urakami S, Shirakawa H, Ishibe T: Relationship of genetic instability with immunoreactivities for p53 protein and proliferating cell nuclear antigen in transitional cell carcinoma of the bladder. Eur Urol. 30:80-88, 1996
  24. Shiina H, Igawa M, Urakami S, Shirakawa H, Ishibe T: Comparison of ureteropelvic transitional cell carcinoma with bladder transitional cell carcinoma using an image analyzer. Urol Int. 56:163-168, 1996
  25. Igawa M, Urakami S, Shiina H, Ishibe T, Matsubara A, Kadena H, Usui T: Limitations of ureteroscopy in diagnosis of invasive upper tract urothelial cancer. Urol Int. 56:13-15, 1996
  26. Igawa M, Urakami S, Shiina H, Ishibe T, Usui T, Chodak GW: Association of nm23 protein levels in human prostates with proliferating cell nuclear antigen expression at autopsy. Eur Urol. 30:383-387, 1996
  27. Igawa M, Urakami S, Shirakawa H, Shiina H, Ishibe T, Kadena H, Usui T, Kawanishi M: Intravesical instillation of epirubicin: effect on tumour recurrence in patients with dysplastic epithelium after transurethral resection of superficial bladder tumour. Br J Urol. 77:358-362, 1996
  28. Shiina H, Igawa M, Yagi H, Urakami S, Yoneda T, Shirakawa H, Ishibe T: Immunohistochemistry of p53 protein in transitional-cell carcinoma of the bladder using an image analyzer. Oncology 53:233-240, 1996
  29. Shiina H, Igawa M, Urakami S, Honda S, Shirakawa H, Ishibe T: Immunohistochemical analysis of bcl-2 expression in transitional cell carcinoma of the bladder. J Clin Pathol. 49:395-399, 1996
  30. Shiina H, Igawa M, Ishibe T: Clinical study on estramustine binding protein (EMBP) in human prostate. Prostate 29:169-176, 1996
  31. Shiina H, Igawa M, Nagami H, Yagi H, Urakami S, Yoneda T, Shirakawa H, Ishibe T, Kawanishi M: Immunohistochemical analysis of proliferating cell nuclear antigen, p53 protein and nm23 protein, and nuclear DNA content in transitional cell carcinoma of the bladder. Cancer 78:1762-1774, 1996
  32. Shiina H, Igawa M, Urakami S, Shirakawa H, Ishibe T, Kawanishi M: Clinical significance of immunohistochemically detectable p53 protein in renal cell carcinoma. Eur Urol. 31:73-80, 1997
  33. Urakami S, Igawa M, Shirakawa H, Shiina H, Kawanishi M: Quantitative analysis of NM23 protein immunoreactivity in renal cell carcinoma using an image analyzer. J Urol. 157:1928-1931, 1997
  34. Shiina H, Igawa M, Shigeno K, Wada Y, Yoneda T, Shirakawa H, Ishibe T, Shirakawa R, Nagasaki M, Shirane T, Usui T: Immunohistochemical analysis of estramustine binding protein with particular reference to proliferative activity in human prostatic carcinoma. Prostate 32:49-58, 1997
  35. Wada Y, Yokogi H, Moriyama-Gonda N, Shigeno K, Shiina H, Igawa M: Chromosome aberrations in renal tumors detected by fluorescence in situ hybridization. Cancer Genet Cytogenet. 99:38-44, 1997
  36. Shiina H, Igawa M, Shigeno K, Wada Y, Yoneda T, Yagi H, Shirakawa R, Nagasaki M: Relationship of serum testosterone level with proliferating cell nuclear antigen and nm23 protein in human prostatic carcinoma tissue. Oncology 54:482-489, 1997
  37. Wada Y, Igawa M, Shiina H, Shigeno K, Yokogi H, Urakami S, Yoneda T, Maruyama R: Comparison of chromosomal aberrations detected by fluorescence in situ hybridization with clinical parameters, DNA ploidy and Ki 67 expression in renal cell carcinoma. Br J Cancer. 77:2003-2007, 1998
  38. Moriyama-Gonda N, Igawa M, Shiina H, Wada Y: Heat-induced membrane damage combined with adriamycin on prostate carcinoma PC-3 cells: correlation of cytotoxicity, permeability and P-glycoprotein or metallothionein expression. Br J Urol. 82:552-559, 1998
  39. Shiina H, Igawa M, Shigeno K, Yamasaki Y, Urakami S, Yoneda T, Wada Y, Honda S, Nagasaki M: Clinical significance of mdm2 and p53 expression in bladder cancer. A comparison with cell proliferation and apoptosis. Oncology 56:239-247, 1999
  40. Moriyama-Gonda N, Igawa M, Shiina H, Urakami S, Wada Y, Terashima M: Heat-induced cellular damage and tolerance in combination with adriamycin for the PC-3 prostate cancer cell line: relationships with cytotoxicity, reactive oxygen species and heat shock protein 70 expression. Eur Urol. 38:235-240, 2000
  41. Shigeno K, Igawa M, Shiina H, Wada H, Yoneda T: The role of colour Doppler ultrasonography in detecting prostate cancer. BJU Int. 86:229-233, 2000
  42. Shiina H, Igawa M, Urakami S, Shigeno K, Yoneda T, Terashima M, Deguchi M, Ribeiro-Filho L, Dahiya R: Alterations of beta- and gamma-catenin in N-butyl-N-(-4-hydroxybutyl) nitrosamine-induced murine bladder cancer. Cancer Res. 61:7101-7109, 2001
  43. Moriyama-Gonda N, Igawa M, Shiina H, Urakami S, Shigeno K, Terashima M: Modulation of heat-induced cell death in PC-3 prostate cancer cells by the antioxidant inhibitor diethyldithiocarbamate. BJU Int. 90:317-325, 2002
  44. Tanaka Y, Sasaki M, Kaneuchi M, Shiina H, Igawa M, Dahiya R: Polymorphisms of the CYP1B1 gene have higher risk for prostate cancer. Biochem Biophys Res Commun. 296:820-826, 2002
  45. Ribeiro-Filho LA, Franks J, Sasaki M, Shiina H, Li LC, Nojima D, Arap S, Carroll P, Enokida H, Nakagawa M, Yonezawa S, Dahiya R: CpG hypermethylation of promoter region and inactivation of E-cadherin gene in human bladder cancer. Mol Carcinog. 34:187-198, 2002
  46. Shiina H, Igawa M, Shigeno K, Terashima M, Deguchi M, Yamanaka M, Ribeiro-Filho L, Kane CJ, Dahiya R: Beta-catenin mutations correlate with over expression of C-myc and cyclin D1 genes in bladder cancer. J Urol. 168:2220-2226, 2002
  47. Yamanaka M, Shirai M, Shiina H, Shirai M, Tanaka Y, Fujime M, Okuyama A, Dahiya R: Loss of anti-apoptotic genes in aging rat crura. J Urol. 168:2296-2300, 2002
  48. Urakami S, Igawa M, Kikuno N, Yoshino T, Kishi H, Shigeno K, Shiina H: Combination chemotherapy with paclitaxel, estramustine and carboplatin for hormone refractory prostate cancer. J Urol. 168:2444-2450, 2002
  49. Li LC, Zhao H, Shiina H, Kane CJ, Dahiya R: PGDB: a curated and integrated database of genes related to the prostate. Nucleic Acids Res. 31:291-293, 2003
  50. Shigeno K, Igawa M, Shiina H, Kishi H, Urakami S: Transrectal colour Doppler ultrasonography for quantifying angiogenesis in prostate cancer. BJU Int. 91:223-226, 2003
  51. Yoneda T, Igawa M, Shiina H, Shigeno K, Urakami S: Postoperative morbidity, functional results and quality of life of patients following orthotopic neobladder reconstruction. Int J Urol. 10:119-125, 2003
  52. Deguchi M, Shiina H, Igawa M, Kaneuchi M, Nakajima K, Dahiya R: DNA mismatch repair genes in renal cell carcinoma. J Urol. 169:2365-2371, 2003
  53. Shiina H, Igawa M, Breault J, Ribeiro-Filho L, Pookot D, Urakami S, Terashima M, Deguchi M, Yamanaka M, Shirai M, Kaneuchi M, Kane CJ, Dahiya R: The human T-cell factor-4 gene splicing isoforms, Wnt signal pathway, and apoptosis in renal cell carcinoma. Clin Cancer Res. 9:2121-2132, 2003
  54. Yamanaka M, Shirai M, Shiina H, Tanaka Y, Tsujimura A, Matsumiya K, Okuyama A, Dahiya R: Diabetes induced erectile dysfunction and apoptosis in penile crura are recovered by insulin treatment in rats. J Urol. 170:291-297, 2003
  55. Tsuchiya D, Hong S, Matsumori Y, Shiina H, Kayama T, Swanson RA, Dillman WH, Liu J, Panter SS, Weinstein PR: Overexpression of rat heat shock protein 70 is associated with reduction of early mitochondrial cytochrome C release and subsequent DNA fragmentation after permanent focal ischemia. J Cereb Blood Flow Metab. 23:718-727, 2003
  56. Kikuno N, Yoshino T, Urakami S, Shigeno K, Kishi H, Hata K, Shiina H, Igawa M: The role of thymidine phosphorylase (TP) mRNA expression in angiogenesis of prostate cancer. Anticancer Res. 23:1305-1312, 2003
  57. Adachi H, Igawa M, Shiina H, Urakami S, Shigeno K, Hino O: Human bladder tumors with 2-hit mutations of tumor suppressor gene TSC1 and decreased expression of p27. J Urol. 170:601-604, 2003
  58. Tanaka Y, Sasaki M, Kaneuchi M, Shiina H, Igawa M, Dahiya R: Polymorphisms of estrogen receptor alpha in prostate cancer. Mol Carcinog. 37:202-208, 2003
  59. Mutaguchi K, Yasumoto H, Mita K, Matsubara A, Shiina H, Igawa M, Dahiya R, Usui T: Restoration of insulin-like growth factor binding protein-related protein 1 has a tumor-suppressive activity through induction of apoptosis in human prostate cancer. Cancer Res. 63:7717-7723, 2003
  60. Shirai M, Yamanaka M, Shiina H, Igawa M, Fujime M, Lue TF, Dahiya R: Downregulation of androgen, estrogen and progesterone receptor genes and protein is involved in aging-related erectile dysfunction. Int J Impot Res. 15:391-396, 2003
  61. Moriyama-Gonda N, Igawa M, Shiina H, Urakami S, Terashima M: Decreased survival of prostate cancer cells in vitro by combined treatment of heat and an antioxidant inhibitor diethyldithiocarbamate (DDC). Exp Toxicol Pathol. 55:251-256, 2003
  62. Adachi H, Majima S, Kon S, Kobayashi T, Kajino K, Mitani H, Hirayama Y, Shiina H, Igawa M, Hino O: Niban gene is commonly expressed in the renal tumors: a new candidate marker for renal carcinogenesis. Oncogene. 23:3495-3500, 2004
  63. Kaneuchi M, Sasaki M, Tanaka Y, Shiina H, Verma M, Ebina Y, Nomura E, Yamamoto R, Sakuragi N, Dahiya R: Expression and methylation status of 14-3-3 sigma gene can characterize the different histological features of ovarian cancer. Biochem Biophys Res Commun. 316:1156-1162, 2004
  64. Kishi H, Igawa M, Kikuno N, Yoshino T, Urakami S, Shiina H: Expression of the survivin gene in prostate cancer: correlation with clinicopathological characteristics, proliferative activity and apoptosis. J Urol. 171:1855-1860, 2004
  65. Enokida H, Shiina H, Igawa M, Ogishima T, Kawakami T, Bassett WW, Anast JW, Li LC, Urakami S, Terashima M, Verma M, Kawahara M, Nakagawa M, Kane CJ, Carroll PR, Dahiya R: CpG hypermethylation of MDR1 gene contributes to the pathogenesis and progression of human prostate cancer. Cancer Res. 64:5956-5962, 2004
  66. Li LC, Shiina H, Deguchi M, Zhao H, Okino ST, Kane CJ, Carroll PR, Igawa M, Dahiya R: Age-dependent methylation of ESR1 gene in prostate cancer. Biochem Biophys Res Commun. 321:455-461, 2004
  67. Kikuno N, Moriyama-Gonda N, Yoshino T, Yoneda T, Urakami S, Terashima M, Yoshida M, Kishi H, Shigeno K, Shiina H, Igawa M: Blockade of paclitaxel-induced thymidine phosphorylase expression can accelerate apoptosis in human prostate cancer cells. Cancer Res. 64:7526-7532, 2004
  68. Kawakami T, Shiina H, Igawa M, Deguchi M, Nakajima K, Ogishima T, Tokizane T, Urakami S, Enokida H, Miura K, Ishii N, Kane CJ, Carroll PR, Dahiya R: Inactivation of the hMSH3 mismatch repair gene in bladder cancer. Biochem Biophys Res Commun. 325:934-942, 2004
  69. Shirai M, Yamanaka M, Shiina H, Igawa M, Ogishima T, Fujime M, Ishii N, Okuyama A, Lue TF, Dahiya R: Androgen, estrogen, and progesterone receptor gene regulation during diabetic erectile dysfunction and insulin treatment. Urology 64: 1244-1249, 2004
  70. Yamanaka M, Shirai M, Shiina H, Tanaka Y, Enokida H, Tsujimura A, Matsumiya K, Okuyama A, Dahiya R: Vascular endothelial growth factor restores erectile function through inhibition of apoptosis in diabetic rat penile crura. J Urol. 173:318-323, 2005
  71. Aoyama K, Burns DM, Suh SW, Garnier P, Matsumori Y, Shiina H, Swanson RA: Acidosis causes endoplasmic reticulum stress and caspase-12-mediated astrocyte death. J Cereb Blood Flow Metab. 25:358-370, 2005
  72. Breault JE, Shiina H, Igawa M, Ribeiro-Filho LA, Deguchi M, Enokida H, Urakami S, Terashima M, Nakagawa M, Kane CJ, Carroll PR, Dahiya R: Methylation of the gamma-catenin gene is associated with poor prognosis of renal cell carcinoma. Clin Cancer Res. 11:557-564, 2005
  73. Ogishima T, Shiina H, Breault JE, Tabatabai L, Bassett WW, Enokida H, Li LC, Kawakami T, Urakami S, Ribeiro-Filho LA, Terashima M, Fujime M, Igawa M, Dahiya R: Increased heparanase expression is caused by promoter hypomethylation and up-regulation of transcriptional factor early growth response-1 in human prostate cancer. Clin Cancer Res. 11:1028-1036, 2005
  74. Urakami S, Yoshino T, Kikuno N, Imai S, Honda S, Yoneda T, Kishi H, Shigeno K, Shiina H, Igawa M: Docetaxel-based chemotherapy as second-line treatment for paclitaxel-based chemotherapy-resistant hormone-refractory prostate cancer: a pilot study. Urology 65:543-548, 2005
  75. Shiina H, Breault JE, Basset WW, Enokida H, Urakami S, Li LC, Okino ST, Deguchi M, Kaneuchi M, Terashima M, Yoneda T, Shigeno K, Carroll PR, Igawa M, Dahiya R: Functional Loss of the gamma-catenin gene through epigenetic and genetic pathways in human prostate cancer. Cancer Res. 65:2130-2138, 2005
  76. Enokida H, Shiina H, Urakami S, Igawa M, Ogishima T, Pookot D, Li LC, Tabatabai ZL, Kawahara M, Nakagawa M, Kane CJ, Carroll PR, Dahiya R: Ethnic group-related differences in CpG hypermethylation of the GSTP1 gene promoter among African-American, Caucasian and Asian patients with prostate cancer. Int J Cancer. 116:174-181, 2005
  77. Zhao H, Shiina H, Greene KL, Li LC, Tanaka Y, Kishi H, Igawa M, Kane CJ, Carroll P, Dahiya R: CpG methylation at promoter site -140 inactivates TGFbeta2 receptor gene in prostate cancer. Cancer 104:44-52, 2005
  78. Youssif M, Shiina H, Urakami S, Gleason C, Nunes L, Igawa M, Enokida H, Tanagho EA, Dahiya R: Effect of vascular endothelial growth factor on regeneration of bladder acellular matrix graft: histologic and functional evaluation. Urology 66:201-207, 2005
  79. Ogishima T, Shiina H, Breault JE, Terashima M, Honda S, Enokida H, Urakami S, Tokizane T, Kawakami T, Ribeiro-Filho LA, Fujime M, Kane CJ, Carroll PR, Igawa M, Dahiya R: Promoter CpG hypomethylation and transcription factor EGR1 hyperactivate heparanase expression in bladder cancer. Oncogene 24:6765-6772, 2005
  80. Tokizane T, Shiina H, Igawa M, Enokida H, Urakami S, Kawakami T, Ogishima T, Okino ST, Li LC, Tanaka Y, Nonomura N, Okuyama A, Dahiya R: Cytochrome P450 1B1 is overexpressed and regulated by hypomethylation in prostate cancer. Clin Cancer Res. 11:5793-5801, 2005
  81. Kaneuchi M, Sasaki M, Tanaka Y, Shiina H, Yamada H, Yamamoto R, Sakuragi N, Enokida H, Verma M, Dahiya R: WT1 and WT1-AS genes are inactivated by promoter methylation in ovarian clear cell adenocarcinoma. Cancer 104:1924-1930, 2005
  82. Enokida H, Shiina H, Urakami S, Igawa M, Ogishima T, Li LC, Kawahara M, Nakagawa M, Kane CJ, Carroll PR, Dahiya R: Multigene methylation analysis for detection and staging of prostate cancer. Clin Cancer Res. 11:6582-6588, 2005
  83. Yoneda T, Adachi H, Urakami S, Kishi H, Shigeno K, Shiina H, Igawa M: Health related quality of life after orthotopic neobladder construction and its comparison with normative values in the Japanese population. J Urol. 174:1944-1947, 2005
  84. Enokida H, Shiina H, Urakami S, Terashima M, Ogishima T, Li LC, Kawahara M, Nakagawa M, Kane CJ, Carroll PR, Igawa M, Dahiya R: Smoking influences aberrant CpG hypermethylation of multiple genes in human prostate carcinoma. Cancer 106:79-86, 2006
  85. Urakami S, Shiina H, Enokida H, Kawakami T, Tokizane T, Ogishima T, Tanaka Y, Li LC, Ribeiro-Filho LA, Terashima M, Kikuno N, Adachi H, Yoneda T, Kishi H, Shigeno K, Konety BR, Igawa M, Dahiya R: Epigenetic inactivation of Wnt inhibitory factor-1 plays an important role in bladder cancer through aberrant canonical Wnt/beta-catenin signaling pathway. Clin Cancer Res. 12:383-391, 2006
  86. Shirai M, Yamanaka M, Shiina H, Igawa M, Kawakami T, Ishii N, Lue TF, Fujime M, Dahiya R: Vascular endothelial growth factor restores erectile function through modulation of the insulin-like growth factor system and sex hormone receptors in diabetic rat. Biochem Biophys Res Commun. 341:755-762, 2006
  87. Tanaka Y, Sasaki M, Shiina H, Tokizane T, Deguchi M, Hirata H, Hinoda Y, Okayama N, Suehiro Y, Urakami S, Kawakami T, Kaneuchi M, Pookot D, Igawa M, Okuyama A, Ishii N, Dahiya R: Catechol-O-methyltransferase gene polymorphisms in benign prostatic hyperplasia and sporadic prostate cancer. Cancer Epidemiol Biomarkers Prev. 15:238-244, 2006
  88. Urakami S, Shiina H, Enokida H, Kawakami T, Kawamoto K, Hirata H, Tanaka Y, Kikuno N, Nakagawa M, Igawa M, Dahiya R: Combination analysis of hypermethylated Wnt-antagonist family genes as a novel epigenetic biomarker panel for bladder cancer detection. Clin Cancer Res. 12:2109-2116, 2006
  89. Okino ST, Pookot D, Li LC, Zhao H, Urakami S, Shiina H, Igawa M, Dahiya R: Epigenetic inactivation of the dioxin-responsive cytochrome P4501A1 gene in human prostate cancer. Cancer Res. 66:7420-7428, 2006
  90. Yoshino T, Shiina H, Urakami S, Kikuno N, Yoneda T, Shigeno K, Igawa M: Bcl-2 expression as  a predictive marker of hormone-refractory prostate cancer treated with taxane-based chemotherapy. Clin Cancer Res. 12:6116-6124, 2006
  91. Shigeno K, Arichi N, Yoneda T, Kishi H, Shiina H, Igawa M: Usefulness of an immunochromatographical assay, PSA Rapid Test as a primary screening test for prostate cancer. Int Urol Nephrol. 38:565-569, 2006
  92. Urakami S, Shiina H, Enokida H, Hirata H, Kawamoto K, Kawakami T, Kikuno N, Tanaka Y, Majid S, Nakagawa M, Igawa M, Dahiya R: Wnt antagonist family genes as biomarkers for diagnosis, staging, and prognosis of renal cell carcinoma using tumor and serum DNA. Clin Cancer Res. 12:6989-6997, 2006
  93. Kikuno N, Urakami S, Nakamura S, Hiraoka T, Hyuga T, Arichi N, Wake K, Sumura M, Yoneda T, Kishi H, Shigeno K, Shiina H, Igawa M: Phase-II study of docetaxel, estramustine phosphate, and carboplatin in patients with hormone-refractory prostate cancer. Eur Urol. 51:1252-1258, 2007
  94. Tanaka Y, Hirata H, Chen Z, Kikuno N, Kawamoto K, Majid S, Tokizane T, Urakami S, Shiina H, Nakajima K, Dhir R, Dahiya R: Polymorphisms of catechol-O-methyltransferase in men with renal cell cancer. Cancer Epidemiol Biomarkers Prev. 16:92-97, 2007
  95. Urakami S, Shiina H, Enokida H, Kawamoto K, Kikuno N, Fandel T, Vejdani K, Nunes L, Igawa M, Tanagho EA, Dahiya R: Functional improvement in spinal cord injury-induced neurogenic bladder by bladder augmentation using bladder acellular matrix graft in the rat. World J Urol. 25:207-213, 2007
  96. Kikuno N, Shiina H, Urakami S, Kawamoto K, Hirata H, Tanaka Y, Place RF, Pookot D, Majid S, Igawa M, Dahiya R: Knockdown of astrocyte-elevated gene-1 inhibits prostate cancer progression through upregulation of FOXO3a activity. Oncogene 26:7647-7655, 2007
  97. Matsubara A, Yoneda T, Yasumoto H, Teishima J, Shiina H, Igawa M, Mita K, Usui T: Lower urinary tract symptoms after radical perineal prostatectomy. Jpn J Clin Oncol. 37:534-539, 2007
  98. Sumura M, Shigeno K, Hyuga T, Yoneda T, Shiina H, Igawa M: Initial evaluation of prostate cancer with real-time elastography based on step-section pathologic analysis after radical prostatectomy: a preliminary study. Int J Urol. 14:811-816, 2007
  99. Matsubara A, Yoneda T, Nakamoto T, Maruyama S, Koda S, Goto K, Teishima J, Shiina H, Igawa M, Usui T: Inguinal hernia after radical perineal prostatectomy: comparison with the retropubic approach. Urology 70:1152-1156, 2007
  100. Moriyama-Gonda N, Shiina H, Terashima M, Satoh K, Igawa M: Rationale and clinical implication of combined chemotherapy with cisplatin and oestrogen in prostate cancer: primary evidence based on methylation analysis of oestrogen receptor-alpha. BJU Int. 101:485-491, 2008
  101. Urakami S, Shiina H, Sumura M, Honda S, Wake K, Hiraoka T, Inoue S, Ishikawa N, Igawa M: Long-term control or possible cure? Treatment of stage D2 prostate cancer under chemotherapy using cisplatin and estramustine phosphate followed by maximal androgen blockade. Int Urol Nephrol. 40:365-368, 2008
  102. Kikuno N, Shiina H, Urakami S, Kawamoto K, Hirata H, Tanaka Y, Majid S, Igawa M, Dahiya R: Genistein mediated histone acetylation and demethylation activates tumor suppressor genes in prostate cancer cells. Int J Cancer. 123:552-560, 2008
  103. Kikuno N, Kawamoto K, Hirata H, Vejdani K, Kawakami K, Fandel T, Nunes L, Urakami S, Shiina H, Igawa M, Tanagho E, Dahiya R: Nerve growth factor combined with vascular endothelial growth factor enhances regeneration of bladder acellular matrix graft in spinal cord injury-induced neurogenic rat bladder. BJU Int. 103:1424-1428, 2009
  104. Arichi N, Kishikawa H, Mitsui Y, Kato T, Nishimura K, Tachikawa R, Tomii K, Shiina H, Igawa M, Ichikawa Y: Cluster outbreak of Pneumocystis pneumonia among kidney transplant patients within a single center. Transplant Proc. 41:170-172, 2009
  105. Inoue S, Shiina H, Hiraoka T, Wake K, Sumura M, Honda S, Urakami S, Igawa M, Usui T: Five-year longitudinal effect of radical perineal prostatectomy on health-related quality of life in Japanese men, using general and disease-specific measures. BJU Int. 104:1077-1084, 2009
  106. Tanaka Y, Zaman MS, Majid S, Liu J, Kawakami K, Shiina H, Tokizane T, Dahiya AV, Sen S, Nakajima K: Polymorphisms of MLH1 in benign prostatic hyperplasia and sporadic prostate cancer. Biochem Biophys Res Commun. 383:440-444, 2009
  107. Inoue S, Shiina H, Hiraoka T, Mitsui Y, Sumura M, Urakami S, Igawa M: Retrospective analysis of the distance between the neurovascular bundle and prostate cancer foci in radical prostatectomy specimens: its clinical implication in nerve-sparing surgery. BJU Int. 104:1085-1090, 2009
  108. Kawakami T, Urakami S, Hirata H, Tanaka Y, Nakajima K, Enokida H, Shiina H, Ogishima T, Tokizane T, Kawamoto K, Miura K, Ishii N, Dahiya R: Superoxide dismutase analog (Tempol: 4-hydroxy-2, 2, 6, 6-tetramethylpiperidine 1-oxyl) treatment restores erectile function in diabetes-induced impotence. Int J Impot Res. 21:348-355, 2009
  109. Inoue S, Shiina H, Sumura M, Urakami S, Matsubara A, Igawa M: Impact of a novel, extended approach of perineal radical prostatectomy on surgical margins in localized prostate cancer. BJU Int. 106:44-48, 2010
  110. Inoue S, Shiina H, Arichi N, Mitsui Y, Hiraoka T, Wake K, Sumura M, Honda S, Yasumoto H, Urakami S, Matsubara A, Igawa M: Identification of lymphatic pathway involved in the spreading of prostate cancer by fluorescence navigation approach with intraoperatively injected indocyanine green. Can Urol Assoc J. 5:254-259, 2011
  111. Mitsui Y, Yasumoto H, Arichi N, Honda S, Shiina H, Igawa M: Current chemotherapeutic strategies against bladder cancer. Int Urol Nephrol. 44:431-441, 2012
  112. Mitsui Y, Shiina H, Hiraki M, Arichi N, Hiraoka T, Sumura M, Honda S, Yasumoto H, Igawa M: Tumor suppressor function of PGP9.5 is associated with epigenetic regulation in prostate cancer -- novel predictor of biochemical recurrence after radical surgery. Cancer Epidemiol Biomarkers Prev. 21:487-496, 2012
  113. Mitsui Y, Shiina H, Arichi N, Hiraoka T, Inoue S, Sumura M, Honda S, Yasumoto H, Igawa M: Indocyanine green (ICG)-based fluorescence navigation system for discrimination of kidney cancer from normal parenchyma: application during partial nephrectomy. Int Urol Nephrol, 44: 753-759, 2012
  114. Mitsui Y, Shiina H, Yamamoto Y, Haramoto M, Arichi N, Yasumoto H, Kitagaki H,Igawa M: Prediction of survival benefit using an automated bone scan index in patients with castration-resistant prostate cancer. BJU International, in press
  115. Mitsui Y, Shiina H, Hiraoka T, Arichi N, Yasumoto H, Dahiya R, Tanagho EA, Igawa M: Simultaneous implantation of bilateral ureters into bladder acellular matrix graft after partial cystectomy in a porcine model. BJU International, 110 : 1212-1217, 2012
  116. Inoue S, Shiina H, Mitsui Y, Yasumoto H, Matsubara A, Igawa M: Identification of lymphatic pathway involved in the spread of bladder cancer:Evidence obtained from fluorescence navigation with intraoperatively injected indocyanine green. Can Urol Assoc J. in press