島根大学医学部眼科学講座
修士課程修了(Aysha Siddika Mukta)
2025.03
Title:
Possible effect of topical rho-kinase inhibitor on Schlemm's canal morphology parameters
Aysha Siddika Mukta, Masaki Tanito, Aika Tsutsui, Sachiko kaidzu, Teruhiko Hamanaka Kanae Kobayashi
Objective:
Ripasudil, a rho-kinase inhibitor, is an ocular hypotensive agent for glaucoma.
The effects of topical ripasudil on Schlemm’s canal (SC) morphology was evaluated histologically
in primary open-angle glaucoma (POAG) patients.
Subjects and Methods:
A total of 182 specimens of anterior chamber gonio tissues were
obtained from 171 eyes of 152 patients during trabeculectomy.
For this study, 95 consecutive specimens were selected based on the following inclusion criteria;
1. The patients had used four components of anti-glaucoma medications
(i.e., FP agonists, β-blockers, CAI, and α2 agonists; non-user group, n=68) or
these four components plus ripasudil (user group, n=27), and 2.
The patients whose preserved tissue sections are available for morphological analyses.
The excised specimen was stained with thrombomodulin (TBM), a marker for endothelial cells of SC,
and SC morphological parameters including total SC length (TSC),
TBM positive and opened SC area (POSC-A) and TBM-negative and opened SC area (NOSC-A) were measured using Image J.
Results:
TSC (p=0.023) was significantly longer in user group (302.5±46.7μm) compared to
non-user group (273.0±59.0 μm). POSC-A (p=0.222) was
equivalent between user group (1857.8±1187.7 μm2) and non-user group (1552.2±1053.7 μm2),
while NOSC-A (p=0.020) was significantly larger in
user group (554.3±665.1 μm2) compared to non-user group (298.6±378.3 μm2).
Conclusion:
The result of the study suggested that preoperative use of ripasudil could potentially
preserve morphological integrity of the lumen of Schlemm's canal in POAG patients.
My impression in Shimane University at department of Ophthalmology:
Dear Professors and Members of the Department of Ophthalmology
As I reach the end of my Master’s journey, I would like to express my deepest gratitude to
Shimane University and especially to the Department of Ophthalmology for your generous support,
kind encouragement, and invaluable guidance .
Studying in Izumo has been a blessing beyond words. This city, with its gentle rhythm, sacred shrines,
and the peaceful embrace of nature, offered not only academic inspiration but also a sense of calm and belonging.
I will always remember walking through the quiet streets during cherry blossom season,
when the soft pink petals floated through the air like a gentle snowfall.
Those moments, filled with beauty and stillness, reflected the kindness and grace I found in the people around me.
I am sincerely thankful to Professor Masaki Tanito-sensei,
whose leadership, patience, and thoughtful guidance helped me navigate both research and life in a new country.
I am equally grateful to Sano-sensei, Aika Tsutsui-sensei, and Sachiko Kaidzu-sensei?
thank you for your continuous support, encouragement, and kindness.
Your warm words and thoughtful advice always gave me strength and confidence.
To all the members of the Department of Ophthalmology, thank you for creating such a welcoming and inspiring environment.
It was a privilege to be a part of this wonderful academic family.
As I move forward in my journey,
I will always carry with me the gentle beauty of Izumo’s cherry blossoms and the wisdom and compassion I found at Shimane University.
With gratitude,
Aysha Siddika Mukta